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AVESTIN Inc
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Microfluidics Inc
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IDEX
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Microfluidics Inc
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Lunaphore
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IDEX
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Ames Research Laboratories
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Lunaphore
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Image Search Results
Journal: Microsystems & Nanoengineering
Article Title: Microfluidics-assisted multiplexed biomarker detection for in situ mapping of immune cells in tumor sections
doi: 10.1038/s41378-019-0104-z
Figure Lengend Snippet: a COC chip with microfluidic channels, a PDMS gasket to create the reaction chamber on top of the tissue sample, and a glass coverslip to enable online imaging of the tissue slide. The exchange of reagents is done in a timeframe of 1 s, following the principle of the fast-fluidic exchange (FFEX) technology. b The LTC is mounted on the stainer and clamped to the glass slide with the tissue by two air-pressure-driven pistons which apply force vertically as indicated by the arrow. It also contains a heating element for precise control of the temperature of the chamber. Furthermore, the stainer can be mounted on a regular motorized microscope stage, thereby enabling the scanning of the tissue slide when clamped with the LTC. c Tissue preprocessing (including dewaxing and antigen retrieval) is performed off-chip. Subsequently, the slide is transferred to the stainer where staining, imaging and elution cycles are performed
Article Snippet: D.G.D. and B.P., at the submission of the paper, are employed at
Techniques: Imaging, Control, Microscopy, Staining
Journal: Microsystems & Nanoengineering
Article Title: Microfluidics-assisted multiplexed biomarker detection for in situ mapping of immune cells in tumor sections
doi: 10.1038/s41378-019-0104-z
Figure Lengend Snippet: a Fluorescence images of biomarkers in tonsils from microfluidic 10-plex IF and bright-field images of conventional single-plex IHC on adjacent slides. Scale bars: 100 µm (overview images) and 15 µm (insets). b Comparison of the stained area fraction from microfluidic multiplex IF and manual single-plex IHC for each marker. The correlation coefficient r and its p value are reported in the same graph
Article Snippet: D.G.D. and B.P., at the submission of the paper, are employed at
Techniques: Fluorescence, Comparison, Staining, Multiplex Assay, Marker